PRIMACEN : Plate-Forme de Recherche en Imagerie Cellulaire de Normandie
Workshop 'Quantification of peptides in biological samples'
2016-07-11 to 2016-07-11 - CURIB, Mont-Saint-Aignan
Organized by David Vaudry, Philippe Chan, Marie Laure Walet Balieu, Luc Arnaud and Olivier Lesouhaitier
Workshop 'Quantification of peptides in biological samples'
CURIB, Rouen University - Mont-Saint-Aignan Campus
July 11th 2016
9 h 00 - 9 h 20 : Registration
9 h 20 - 9 h 30 : Introduction of the day
9 h 30 - 10 h 10 : Conference 1: Discovery, design and delivery of assays to quantify protein/peptide biomarkers Steve Pennington (School of Medicine and Medical Science Conway Institute, University College Dublin, Dublin, Ireland)
10 h 10 - 10 h 50 : Conference 2: MS-based clinical proteomics: requirements for adoption as routine tests Christa Cobbaert (Leiden University Medical Centre, Department of Clinical Chemistry and Laboratory Medicine, The Netherlands)
10 h 50 - 11 h 10 : Coffee break
11 h 10 - 11 h 50 : Conference 3: Sample preparation : an important step for peptide quantification by mass spectrometryMarie Laure Walet Balieu (PISSARO Proteomics Platform, Rouen University, France)
11 h 50 - 12 h 30 : Conference 4: Biomolecule analytics using microscale thermophoresis (MST)François-Xavier Ogi (NanoTemper Technologies)
12:30 - 14:30 : lunch around posters and exhibitions
14:30 - 17:30 : Practical demonstrations (6 people / group)
- Demo on Agilent 6550 Q-TOFPhilippe Chan (PISSARO, Rouen)
- Demo of biomolecular interactions by MicroScale Thermophoresis (MST)François-Xavier Ogi (NanoTemper Technologies)
- Visit of the PRIMACEN Cell Imaging Platform (Peptide synthesis and screening Bioimaging electron microscopy Microdissection and real time PCRDavid Vaudry (PRIMACEN, Rouen)
Additional information regarding the workshop
This workshop will include some conferences in the morning, a lunch around posters and exhibitions and some practical demonstration in the afternoon. Participants will be requested to select 3 practical demonstrations and will be able to assist to 2 of them in the afternoon. Information regarding the presentation on biomolecular interactions by MicroScale Thermophoresis (MST) The workshop will give an overview of Microscale Thermophoresis (MST), a technology for the measurement of biomolecule interactions. MST refers to the directed movement of molecules in optically generated microscopic temperature gradients. This thermophoretic movement is determined by the entropy of the hydration shell around the molecules. Almost all interactions between molecules and virtually any biochemical process are linked to a change in size, charge and/or conformation of molecules which alter this hydration shell and therefore can be detected and quantified by MST. During the demonstration, some positive controls will be measured. People who would like to test their own samples, should contact Mr François-Xavier Ogi (Francois.Ogi@nanotemper.de).
To register to the RegPep2016, please visit our website: www.regpep2016.fr